Ntawm no yog cov kauj ruam los npaj dos slide specimens:
Npaj cov ntaub ntawv thiab cov cuab yeej:
Cov dos tshiab
Microscope slides thiab cover slips
Scalpel los yog txiab
Oxalic acid tov (xaiv tau, rau cov cell softening)
Iodine tov (yeem, rau staining)
Dos sectioning:
Noj cov dos tshiab thiab tshem tawm cov hauv paus hniav uas siv scalpel los yog txiab.
Txiav cov dos rau hauv nyias ntu. Koj tuaj yeem siv scalpel lossis txiab los ua nyias, hla ntu ntawm cov dos. Sim ua kom cov hlais nyias thiab sib xws li sai tau.
soften lub dos hlwb (yeem):
Yog tias koj xav ua kom cov hlwb kom pom kev zoo dua, koj tuaj yeem tso cov dos hauv cov tshuaj oxalic acid rau ob peb feeb. Cov kauj ruam no yog xaiv tau thiab tuaj yeem hla yog tias koj nyiam saib cov dos hlwb hauv lawv lub xeev ntuj.
Hloov cov dos seem mus rau ib tug swb:
Siv lub tshuab kuaj kab mob kom huv si thiab tso dej los yog ib qho kev poob ntawm cov tshuaj oxalic acid (yog siv) rau ntawm nws.
Ua tib zoo hloov cov dos seem mus rau qhov swb siv forceps lossis rab koob.
Maj mam nias rau ntawm cov dos seem nrog lub hau npog kom nws flatten thiab tshem tawm cov npuas npuas.
Kev xaiv staining (iodine tov):
Yog tias koj xav kom stain lub dos hlwb los txhim kho lawv txoj kev pom, koj tuaj yeem tso ib qho iodine tov rau ntawm ntug ntawm daim npog npog. Capillary kev txiav txim yuav kos cov tshuaj iodine hauv qab daim npog daim npog.
Cia lub swb zaum ob peb feeb kom tso cai rau cov tshuaj iodine nkag mus rau hauv cov hlwb.
Tshem tawm cov kua dej ntau dhau:
Tom qab staining (yog tias ua tau) lossis yog tias muaj kua ntau dhau ntawm qhov swb, ua tib zoo txhuam cov kua ntau dhau ntawm lub npog npog nrog cov ntaub so ntswg lossis cov ntawv lim. Ceev faj tsis txhob dislodge cov dos seem.
Mounting swb:
Siv ib qho me me ntawm qhov nruab nrab nruab nrab (xws li glycerin lossis immersion oil) nyob ib ncig ntawm cov npoo ntawm lub hau npog kom tsis txhob ziab thiab khaws cov qauv.
Ntxuav cov swb yog tias tsim nyog, tshem tawm cov ntiv tes lossis cov smudges.
Koj cov dos swb cov qauv tam sim no npaj rau kev soj ntsuam raws li lub tshuab tsom. Muab qhov kev npaj swb rau ntawm lub tshuab kuaj kab mob theem, kho qhov ua kom pom tseeb, thiab tshawb xyuas lub ntiaj teb txaus nyiam ntawm dos hlwb.